LOCUS       40924_40412        4173 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector psiLentGene Puromycin, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Virdugirene J.
  TITLE     siLentGene U6 Hairpin Cloning Systems Technical Manual #TM247
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Virdugirene J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(140..158)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(176..192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(200..216)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(224..254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(269..290)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        465..822
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             851..1447
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    1495..1543
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(1736..2324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2498..3355)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3356..3460)
                     /label=AmpR promoter
     rep_origin      complement(3538..3993)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4134..4150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4157..4173
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt
      121 ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
      181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgctgatctg cgcagcacca
      421 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
      481 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
      541 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      601 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      661 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      721 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
      781 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac
      841 gctagccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
      901 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
      961 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     1021 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     1081 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     1141 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     1201 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     1261 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     1321 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     1381 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     1441 cggtgcctga ctgcaggatc ctcgagttta aactctagaa ccggtcatgg ccgcaataaa
     1501 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac tagaagcttg
     1561 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1621 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1681 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2161 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2341 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2401 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2461 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2521 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2581 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2641 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2701 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2761 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2821 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2881 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2941 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3001 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3061 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3121 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3181 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3241 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3301 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3361 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3421 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3481 cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     3541 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3601 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3661 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3721 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3781 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3841 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3901 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3961 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     4021 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     4081 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     4141 gacggccagt gaattgtaat acgactcact ata
//
